Stem cell culture media, cell culture substrates, and other reagents we have selected to give the best results when used in conjunction with our Quick-Tissue™ stem cell differentiation kits.
iPSC Media & Substrates
iPSC Media, Cell Culture Substrates, & More
Use the right reagents to get the best results with our differentiation kits. The StemFit® Basic series of iPSC media from Ajinomoto Co. is highly cost-effective and supports the expansion and maintenance of undifferentiated stem cells. iMatrix-511 silk from Nippi, Inc. are stem cell culture substrates that provide strong adhesion and support long-term self-renewal of stem cells. We also sell our own coating and dissociation reagents sized to match our differentiation kits for researchers not wanting to invest in full sized reagents for pilot studies.
- Optimized for use with our kits
- Support growth of undifferentiated PSCs
- Xeno- and feeder-free iPSC media
Frequently Asked Questions
How long do I need to maintain hPSCs prior to beginning to use your kit?
Before beginning differentiation, cultures should be healthy and displaying typical morphologies (i.e., round colonies with tightly packed cells exhibiting a small cytoplasmic area), few spontaneously differentiating cells, and normal growth rates (i.e., doubling once a day). We recommend growing the cells for at least 14 days post-thaw before differentiating and not allowing the cultures to reach more than 80% confluency.
Why do differentiation kits require hPSCs be maintained under StemFit or StemFlex conditions?
hPSCs should be adapted to passaging as single cells prior to differentiation. Our protocols have only been tested with StemFit/StemFlex, so we can't guarantee results using other iPSC media.
Can I use Geltrex or Matrigel as a coating substrate for hPSC cultures?
Our kits are not optimized with these substrates. However, if your hPSC differentiation protocols are already optimized with such a substrate, you may try using one of these with our kit at your own risk.