Cholinergic Neurons

Gain a deeper understanding of the complexities of neuronal signaling and tease apart variations due to age, gender, and donor variability with neurons derived from human iPS cells.

  • Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons give you access to the same cells with every order for more biologically relevant and reproducible studies
  • Quick-Neuron™ Cholinergic Kits provide you with a quick and easy way to turn your iPS cells into cholinergic neurons.

Advantages

  • ~ 1 week differentiation
  • Functionally validated
  • Highly pure population
  • No genetic footprint
  • Differentiation Kits and Differentiated Cells available
  • Multiple donors and disease types available

Characterization

Differentiation kit workflow (upper: SeV kit, lower: mRNA kit)

Representative images of Quick-Neuron Cholinergic - SeV Kit cell cultures on days 1-6 post-differentiation (scale bar = 100 μm).

iPSC-derived cells workflow

Representative images of Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons on days 1-3 post-thaw (scale bar = 100 μm).

iPSC-derived cholinergic neurons express neuronal markers and display typical neurite growth. Immunofluorescent staining of Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons on Day 3 post-thaw that shows expression of the pan-neuronal marker TUBB3 and the cholinergic neuron marker ChAT (scale bar = 100 μm).

ALS Disease Study
Healthy control and ALS patient-derived iPSCs were differentiated into cholinergic neurons. The cells were fixed at day 10 from iPSCs and stained by TUBB3 (Green) and TDP-43 (Red).

Neurite length analysis

Our fast differentiation enables quicker and quantitative comparison between healthy and patient or treated and non-treated cultures.

Cholinergic Neuron Differentiation Kits

Our Quick-Neuron™ Cholinergic - SeV and mRNA Kits (small and large sizes available) allow researchers to quickly, easily, and efficiently differentiate their iPS or ES cell line of choice into cholinergic neurons. The kits utilize temperature sensitive Sendai virus and synthetic messenger RNA (syn-mRNA) to deliver our proprietary cocktail of transcription factors that induce differentiation without leaving a genetic footprint.

Cat No. Product Name Disease StatusDescription
CH-SeV Quick-Neuron™ Cholinergic - SeV Kit This kit differentiates human pluripotent stem cells into cholinergic neurons in 6 days using Sendai virus.
CH-mRNA Quick-Neuron™ Cholinergic - mRNA Kit This kit differentiates human pluripotent stem cells into cholinergic neurons in 7 days using synthetic mRNA.
CH-MM Quick-Neuron™ Cholinergic - Maintenance Medium Medium for long-term maintenance of human iPS/ESC-derived cholinergic neurons.

iPSC-derived Cholinergic Neurons

Elixirgen Scientific’s Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons are created using human iPS cells made available through a license from The California Institute for Regenerative Medicine (CIRM).

The CIRM iPS cell repository includes over 1,500 iPS cell lines, so please contact us if you would like cholinergic neurons derived from a specific donor cell line. Every lot of iPSCs is differentiated into cholinergic neurons using our proprietary transcription factor-based technology that is “footprint-free” and delivers functional, high-quality cells.

Cat No. Product Name Disease StatusDescription
CH-SeV-CW50065 Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons (F, 74 yr donor) - Healthy Control Healthy Control Cryopreserved human iPSC-derived cholinergic neurons from healthy control (F, 74)
CH-SeV-CW70067 Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons (F, 21 yr donor) - Healthy Control Healthy Control Cryopreserved human iPSC-derived cholinergic neurons from healthy control (F, 21)
CH-SeV-CW50023 Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons (M, 69 yr donor) - Healthy Control Healthy Control Cryopreserved human iPSC-derived cholinergic neurons from healthy control (M, 69)
CH-SeV-CW10149 Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons (M, 38 yr donor) - Healthy Control Healthy Control Cryopreserved human iPSC-derived cholinergic neurons from healthy control (M, 38)
CH-SeV-CW20300 Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons (M, 10 yr donor) - Healthy Control Healthy Control Cryopreserved human iPSC-derived cholinergic neurons from healthy control (M, 10)
CH-SeV-CW50025 Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons (F, 68 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived cholinergic neurons from Alzheimer's Disease patient (F, 68)
CH-SeV-CW50113 Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons (M, 68 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived cholinergic neurons from Alzheimer's Disease patient (M, 68)
CH-SeV-CW50114 Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons (F, 72 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived cholinergic neurons from Alzheimer's Disease patient (F, 72)
CH-SeV-CW50115 Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons (F, 67 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived cholinergic neurons from Alzheimer's Disease patient (F, 67)
CH-SeV-CW50137 Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons (F, 70 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived cholinergic neurons from Alzheimer's Disease patient (F, 70)
CH-SeV-CW50147 Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons (M, 71 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived cholinergic neurons from Alzheimer's Disease patient (M, 71)
CH-SeV-CW50104 Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons (M, 79 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived cholinergic neurons from Alzheimer's Disease patient (M, 79)
CH-SeV-CW50107 Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons (M, 72 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived cholinergic neurons from Alzheimer's Disease patient (M, 72)
CH-SeV-CW50169 Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons (F, 68 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived cholinergic neurons from Alzheimer's Disease patient (F, 68)
CH-SeV-CW50170 Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons (F, 64 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived cholinergic neurons from Alzheimer's Disease patient (F, 64)
CH-SeV-CW50173 Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons (F, 74 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived cholinergic neurons from Alzheimer's Disease patient (F, 74)
CH-SeV-CW50174 Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons (F, 76 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived cholinergic neurons from Alzheimer's Disease patient (F, 76)
CH-SeV-CW50175 Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons (F, 77 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived cholinergic neurons from Alzheimer's Disease patient (F, 77)
CH-SeV-CW50176 Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons (M, 74 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived cholinergic neurons from Alzheimer's Disease patient (M, 74)
CH-SeV-CW50177 Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons (F, 85 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived cholinergic neurons from Alzheimer's Disease patient (F, 85)
CH-SeV-CW60130 Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons (F, 6 yr donor) - Epilepsy Epilepsy Cryopreserved human iPSC-derived cholinergic neurons from Epilepsy patient (F, 6)
CH-SeV-CW60231 Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons (M, 6 yr donor) - Epilepsy Epilepsy Cryopreserved human iPSC-derived cholinergic neurons from Epilepsy patient (M, 6)
CH-SeV-CW60236 Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons (F, 11 yr donor) - Epilepsy Epilepsy Cryopreserved human iPSC-derived cholinergic neurons from Epilepsy patient (F, 11)
CH-SeV-CW20026 Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons (M, 10 yr donor) - Autism Spectrum Disorder Autism Spectrum Disorder Cryopreserved human iPSC-derived cholinergic neurons from Autism Spectrum Disorder patient (M, 10)
CH-SeV-CW20090 Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons (F, 18 yr donor) - Autism Spectrum Disorder Autism Spectrum Disorder Cryopreserved human iPSC-derived cholinergic neurons from Autism Spectrum Disorder patient (F, 18)
CH-SeV-CW10130 Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons (M, 36 yr donor) - Fatty Liver Disease Fatty Liver Disease Cryopreserved human iPSC-derived cholinergic neurons from Fatty Liver Disease patient (M, 36)

Frequently Asked Questions

Do I need a license to use Elixirgen’s iPSC-derived cells and/or differentiation reagent kits?

No. The use of differentiated cells or kits provided by Elixirgen Scientific does not require any additional license from other parties for any type of use, except for use in humans or for therapeutic or diagnostic use. Check out our license statement in Resource section for further detail.

What sizes of Quick-Neuron™ Excitatory - Human iPSC-derived Neurons are available?

Off the shelf, we offer small (>1 million viable cryopreserved cells) and large (5x >1 million viable cryopreserved cells) sizes. If you are looking for a larger quantity, please contact us for pricing information.

Should I use an mRNA- or SeV-based differentiation kit?

Both delivery methods provide robust “footprint-free,” non-integrating expression of the transcription factors, although each kit uses a slightly different workflow. Based on our data, the differentiation efficiency between an mRNA-based and a SeV-based kit is similar. For example, the percentages of TUBB3+ neurons differentiated using Quick-Neuron™ Cholinergic mRNA- and SeV-based kits were 93% and 88%, respectively.

We recommend the mRNA-based neuron differentiation kits for labs with experience culturing iPSCs/ESCs, as the workflow involves slightly more manipulation of fragile differentiating cells than the SeV-based kits. The SeV-based kits are better for labs with little experience culturing iPSCs/ESCs, although they must be used with BSL-2 safety measures and may require prior approval from your Institutional Biosafety Committee (IBC).

What sizes of Quick-Neuron™ differentiation kits are available?

Off the shelf, we offer small and large iPSC neuronal differentiation kits. The small size is best suited for single cell, imaging analyses, and pilot studies while the large size is best for applications such as high throughput screening and RNA sequencing.