Astrocytes

Human induced pluripotent stem cells (iPSCs) are a powerful tool for studying glial cells in vitro. Human iPS cells overcome many of the limitations of other popular models, such as immortalized cell lines and primary astrocytes, which can be plagued by reproducibility issues and lack of biological relevance. Although human iPS cells have many advantages over existing models, one drawback is that differentiation into the desired cell type is a time and labor-intensive process. To address this research bottleneck, Elixirgen Scientific provides a variety of products and services to make research more efficient. Our proprietary technology allows for rapid, reproducible differentiation of human iPSC-derived astrocytes without sacrificing purity.

iPSC-derived astrocytes from Healthy and Affected Donors

Elixirgen Scientific’s human iPSC-derived astrocyte cells are created using human iPS cells made available through a license from The California Institute for Regenerative Medicine (CIRM).

All of our iPSC-derived astrocytes are differentiated using our proprietary technology that utilizes a potent cocktail of key transcription factors to transform iPSCs into high-quality, differentiated astrocytes. The CIRM iPS cell repository includes over 1,500 iPS cell lines, so please contact us if you would like astrocytes derived from a specific donor cell line.

Advantages

  • High similarity to human primary astrocytes
  • No genetic footprint
  • Multiple donors and disease types to choose from

Cat No. Product Name Disease StatusDescription
AS-SeV-CW50065 Quick-Glia™ Astrocytes - Human iPSC-derived Astrocytes (F, 74 yr donor) - Healthy Control Healthy Control Cryopreserved human iPSC-derived astrocytes from healthy control (F, 74)
AS-SeV-CW70067 Quick-Glia™ Astrocytes - Human iPSC-derived Astrocytes (F, 21 yr donor) - Healthy Control Healthy Control Cryopreserved human iPSC-derived astrocytes from healthy control (F, 21)
AS-SeV-CW50023 Quick-Glia™ Astrocytes - Human iPSC-derived Astrocytes (M, 69 yr donor) - Healthy Control Healthy Control Cryopreserved human iPSC-derived astrocytes from healthy control (M, 69)
AS-SeV-CW10149 Quick-Glia™ Astrocytes - Human iPSC-derived Astrocytes (M, 38 yr donor) - Healthy Control Healthy Control Cryopreserved human iPSC-derived astrocytes from healthy control (M, 38)
AS-SeV-CW20300 Quick-Glia™ Astrocytes - Human iPSC-derived Astrocytes (M, 10 yr donor) - Healthy Control Healthy Control Cryopreserved human iPSC-derived astrocytes from healthy control (M, 10)
AS-SeV-CW50025 Quick-Glia™ Astrocytes - Human iPSC-derived Astrocytes (F, 68 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived astrocytes from Alzheimer's Disease patient (F, 68)
AS-SeV-CW50113 Quick-Glia™ Astrocytes - Human iPSC-derived Astrocytes (M, 68 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived astrocytes from Alzheimer's Disease patient (M, 68)
AS-SeV-CW50114 Quick-Glia™ Astrocytes - Human iPSC-derived Astrocytes (F, 72 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived astrocytes from Alzheimer's Disease patient (F, 72)
AS-SeV-CW50115 Quick-Glia™ Astrocytes - Human iPSC-derived Astrocytes (F, 67 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived astrocytes from Alzheimer's Disease patient (F, 67)
AS-SeV-CW50137 Quick-Glia™ Astrocytes - Human iPSC-derived Astrocytes (F, 70 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived astrocytes from Alzheimer's Disease patient (F, 70)
AS-SeV-CW50147 Quick-Glia™ Astrocytes - Human iPSC-derived Astrocytes (M, 71 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived astrocytes from Alzheimer's Disease patient (M, 71)
AS-SeV-CW50104 Quick-Glia™ Astrocytes - Human iPSC-derived Astrocytes (M, 79 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived astrocytes from Alzheimer's Disease patient (M, 79)
AS-SeV-CW50107 Quick-Glia™ Astrocytes - Human iPSC-derived Astrocytes (M, 72 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived astrocytes from Alzheimer's Disease patient (M, 72)
AS-SeV-CW50169 Quick-Glia™ Astrocytes - Human iPSC-derived Astrocytes (F, 68 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived astrocytes from Alzheimer's Disease patient (F, 68)
AS-SeV-CW50170 Quick-Glia™ Astrocytes - Human iPSC-derived Astrocytes (F, 64 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived astrocytes from Alzheimer's Disease patient (F, 64)
AS-SeV-CW50173 Quick-Glia™ Astrocytes - Human iPSC-derived Astrocytes (F, 74 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived astrocytes from Alzheimer's Disease patient (F, 74)
AS-SeV-CW50174 Quick-Glia™ Astrocytes - Human iPSC-derived Astrocytes (F, 76 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived astrocytes from Alzheimer's Disease patient (F, 76)
AS-SeV-CW50175 Quick-Glia™ Astrocytes - Human iPSC-derived Astrocytes (F, 77 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived astrocytes from Alzheimer's Disease patient (F, 77)
AS-SeV-CW50176 Quick-Glia™ Astrocytes - Human iPSC-derived Astrocytes (M, 74 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived astrocytes from Alzheimer's Disease patient (M, 74)
AS-SeV-CW50177 Quick-Glia™ Astrocytes - Human iPSC-derived Astrocytes (F, 85 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived astrocytes from Alzheimer's Disease patient (F, 85)
AS-SeV-CW60130 Quick-Glia™ Astrocytes - Human iPSC-derived Astrocytes (F, 6 yr donor) - Epilepsy Epilepsy Cryopreserved human iPSC-derived astrocytes from Epilepsy patient (F, 6)
AS-SeV-CW60231 Quick-Glia™ Astrocytes - Human iPSC-derived Astrocytes (M, 6 yr donor) - Epilepsy Epilepsy Cryopreserved human iPSC-derived astrocytes from Epilepsy patient (M, 6)
AS-SeV-CW60236 Quick-Glia™ Astrocytes - Human iPSC-derived Astrocytes (F, 11 yr donor) - Epilepsy Epilepsy Cryopreserved human iPSC-derived astrocytes from Epilepsy patient (F, 11)
AS-SeV-CW20026 Quick-Glia™ Astrocytes - Human iPSC-derived Astrocytes (M, 10 yr donor) - Autism Spectrum Disorder Autism Spectrum Disorder Cryopreserved human iPSC-derived astrocytes from Autism Spectrum Disorder patient (M, 10)
AS-SeV-CW20090 Quick-Glia™ Astrocytes - Human iPSC-derived Astrocytes (F, 18 yr donor) - Autism Spectrum Disorder Autism Spectrum Disorder Cryopreserved human iPSC-derived astrocytes from Autism Spectrum Disorder patient (F, 18)
AS-SeV-CW10130 Quick-Glia™ Astrocytes - Human iPSC-derived Astrocytes (M, 36 yr donor) - Fatty Liver Disease Fatty Liver Disease Cryopreserved human iPSC-derived astrocytes from Fatty Liver Disease patient (M, 36)

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Quick-Glia™ Astrocyte Differentiation Kits & Media

Our Quick-Glia™ Astrocyte differentiation kits allow researchers to quickly, easily, and efficiently differentiate their iPS cell line into astrocytes. The kit uses Sendai virus to over-express transcription factors to iPS cells and does not leave a genetic footprint.

Cat No. Product Name Disease StatusDescription
AS-SeV Quick-Glia™ Astrocyte - SeV Kit Kit for differentiation of human iPS/ES cells into astrocytes in 28 days using Sendai virus.

Characterization

Immunofluorescent staining of Quick-Glia™ Astrocyte shows expression of astrocytic markers CD44, ALDH1L1, and GFAP. Nuclei are counterstained with Hoechst 33342 (cyan) (scale bar = 100 μm). All images are pseudo-colored.

(A) Gene expression profiles of iPSCs and Quick-Glia™ – Astrocyte SeV Culture on day 28 were compared with the profile of human primary astrocytes and the results are shown as scatter plots. The horizontal axis indicates the expression levels of genes in human primary astrocytes purchased from ScienCell (Catalog Number: 1800-5), whereas the vertical axis indicates the expression levels of genes in iPSCs (left) and in Quick-Glia™ – Astrocyte SeV Culture on day 28 (right). The levels of gene expression are shown based on transcripts per million (TPM) in the log10 scale. Blue and green dots represent upregulated and downregulated genes (FDR<0.05), respectively, relative to their levels in human primary astrocytes. (B) Similarities of gene expression profiles of human iPSCs and Quick-Glia™ – Astrocyte SeV Culture on days 14, 28 and 42 to the profile of human primary astrocytes are shown as a bar chart. The vertical axis indicates Pearson correlation (r) based on median-subtracted logTPM.

Real-time quantitative PCR analysis of expression levels of astrocyte-associated genes CD44GFAPS100β and ALDH1L1 were examined. Graphs show comparison of Quick-Glia™ – Astrocyte SeV Culture on  day 28 (A) and day 42 (B) with human brain total RNA (TaKaRa, Catalog Number: 636530). The relative gene expression is normalized to phosphoglycerate kinase 1 (PGK1), and then calculated as a fold induction relative to undifferentiated hPSCs as a control. Error bars show standard deviation.

Frequently Asked Questions

Do I need a license to commercialize products using Elixirgen’s iPSC astrocyte cells?

No. The use of differentiated cells provided by Elixirgen Scientific does not require any additional license from other parties for any type of use, except for use in humans or for therapeutic or diagnostic use.

What sizes of the Quick-Glia™ Astrocyte – SeV Kit are available?

Off the shelf, we offer a set of reagents for use with a total of 4 wells of a 24-well plate and 6 wells of a 6-well plate. If you are looking for a larger quantity, please contact us for pricing information.