Excitatory Neurons

Gain a deeper understanding of the complexities of neuronal signaling and tease apart variations due to age, gender, and donor variability with neurons derived from human iPS cells.

  • Quick-Neuron™ Excitatory - Human iPSC-derived Neurons give you access to the same cells with every order for more biologically relevant and reproducible studies.
  • Quick-Neuron™ Excitatory Kits provide you with a quick and easy way to turn your iPS cells into excitatory neurons.

Advantages

  • ~ 1 week differentiation
  • Functionally validated
  • Highly pure population
  • No genetic footprint
  • Differentiation Kits and Differentiated Cells available
  • Multiple donors and disease types available

Characterization

Differentiation kit workflow (upper: SeV kit, lower: mRNA kit)

Representative images of Quick-NeuronExcitatory - SeV Kit cell cultures on days 1-10 post-differentiation (scale bar = 100 μm).

iPSC-derived cells workflow

Microscope images of iPSC-derived Neuron differentiation over 7 days

Representative images of Quick-Neuron™ Excitatory - Human iPSC-derived Neurons on days 1-7 post-thaw (scale bar = 100 μm).

iPSC-derived excitatory neurons express neuronal markers and display typical neurite growth. Immunofluorescent staining of Quick-Neuron™ Excitatory - Human iPSC-derived Neurons on Day 7 post-thaw that shows expression of the pan-neuronal marker TUBB3 and the glutamatergic neuron marker vGLUT1 (scale bar = 100 μm).

Electrophysiological properties of iPSC-derived excitatory neurons. Whole-cell patch clamp of Quick-Neuron™ Excitatory - Human iPSC-derived Neurons 5-6 weeks post-thaw. (A) A brightfield image of the neurons measured. (B) Spontaneous action potentials were recorded. (C) Spontaneous excitatory postsynaptic current of neurons was detected by voltage clamp measurement at -70mV, indicating the formation of mature synapses. Data courtesy of E-PHY SCIENCE SAS.

iPSC-derived Neurons seeded onto MEA plates remain functional after transport.

Excitatory Neurons seeded onto MEA plates remain functional after transport. Baseline readings of the same well (A) on day 49 post-thaw before transport and (B) on day 50 after 33 hr transport of an MEA plate seeded with Quick-Neuron™ Excitatory Neurons and primary human astrocytes from Tokyo, Japan to Maryland, USA. (C) Response on day 51 to varying concentrations of 4-AP after transport. Data courtesy of Ricoh.

Gene Expression of Quick-Neuron Excitatory Neurons. A heat map of selected gene expression data from RNA-seq performed on undifferentiated iPSCs, Quick-Neuron™ Excitatory Neurons (EX only) cultured for 10 days and 38 days,  Quick-Neuron™ Excitatory Neurons cocultured with primary astrocytes (EX+AST) for 18 and 52 days, and primary fetal and adult brain samples is shown. Values represent log10(TPM + 1). Data courtesy of Ricoh.

Quick-Neuron Excitatory Neurons display gene expression profiles similar to those of human brain: RNA-seq was performed on undifferentiated iPSCs, Quick-Neuron™Excitatory Neurons (EX only) cultured for 10 days and 38 days, Excitatory neurons cocultured with primary astrocytes (EX+AST) for 18 and 52 days, and primary fetal and adult brain samples. Principal component analysis indicates that Quick-Neuron™ Excitatory Neurons display gene expression similar to that of human brain, particularly when grown with astrocytes. As cells remain in culture over time they more closely resemble adult human brain cells. Data courtesy of Ricoh.

Excitatory Neuron Differentiation Kits

Our Quick-Neuron™ Excitatory - SeV and mRNA Kits (small and large sizes available) allow researchers to quickly, easily, and efficiently differentiate their iPS or ES cell line of choice into excitatory neurons. The kits utilize temperature sensitive Sendai virus and synthetic messenger RNA (syn-mRNA) to deliver our proprietary cocktail of transcription factors that induce differentiation without leaving a genetic footprint.

Cat No. Product Name Disease StatusDescription
EX-SeV Quick-Neuron™ Excitatory - SeV Kit This kit differentiates human pluripotent stem cells into excitatory neurons in 10 days using Sendai virus.
EX-mRNA Quick-Neuron™ Excitatory- mRNA Kit This kit differentiates human pluripotent stem cells into excitatory neurons in 10 days using synthetic mRNA.
EX-MM Quick-Neuron™ Excitatory - Maintenance Medium Medium for long-term maintenance of human iPS/ESC-derived Excitatory neurons.

iPSC-derived Excitatory Neurons

Elixirgen Scientific’s Quick-Neuron™ Excitatory - Human iPSC-derived Neurons are created using human iPS cells made available through a license from The California Institute for Regenerative Medicine (CIRM).

The CIRM iPS cell repository includes over 1,500 iPS cell lines, so please contact us if you would like excitatory neurons derived from a specific donor cell line. Every lot of iPSCs is differentiated into excitatory neurons using our proprietary transcription factor-based technology that is “footprint-free” and delivers functional, high-quality cells.

Cat No. Product Name Disease StatusDescription
EX-SeV-CW50065 Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (F, 74 yr donor) - Healthy Control Healthy Control Cryopreserved human iPSC-derived excitatory neurons from healthy control (F, 74)
EX-SeV-CW70067 Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (F, 21 yr donor) - Healthy Control Healthy Control Cryopreserved human iPSC-derived excitatory neurons from healthy control (F, 21)
EX-SeV-CW50023 Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (M, 69 yr donor) - Healthy Control Healthy Control Cryopreserved human iPSC-derived excitatory neurons from healthy control (M, 69)
EX-SeV-CW10149 Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (M, 38 yr donor) - Healthy Control Healthy Control Cryopreserved human iPSC-derived excitatory neurons from healthy control (M, 38)
EX-SeV-CW20300 Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (M, 10 yr donor) - Healthy Control Healthy Control Cryopreserved human iPSC-derived excitatory neurons from healthy control (M, 10)
EX-SeV-CW50025 Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (F, 68 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived excitatory neurons from Alzheimer's Disease patient (F, 68)
EX-SeV-CW50113 Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (M, 68 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived excitatory neurons from Alzheimer's Disease patient (M, 68)
EX-SeV-CW50114 Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (F, 72 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived excitatory neurons from Alzheimer's Disease patient (F, 72)
EX-SeV-CW50115 Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (F, 67 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived excitatory neurons from Alzheimer's Disease patient (F, 67)
EX-SeV-CW50137 Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (F, 70 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived excitatory neurons from Alzheimer's Disease patient (F, 70)
EX-SeV-CW50147 Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (M, 71 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived excitatory neurons from Alzheimer's Disease patient (M, 71)
EX-SeV-CW50104 Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (M, 79 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived excitatory neurons from Alzheimer's Disease patient (M, 79)
EX-SeV-CW50107 Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (M, 72 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived excitatory neurons from Alzheimer's Disease patient (M, 72)
EX-SeV-CW50169 Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (F, 68 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived excitatory neurons from Alzheimer's Disease patient (F, 68)
EX-SeV-CW50170 Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (F, 64 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived excitatory neurons from Alzheimer's Disease patient (F, 64)
EX-SeV-CW50173 Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (F, 74 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived excitatory neurons from Alzheimer's Disease patient (F, 74)
EX-SeV-CW50174 Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (F, 76 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived excitatory neurons from Alzheimer's Disease patient (F, 76)
EX-SeV-CW50175 Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (F, 77 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived excitatory neurons from Alzheimer's Disease patient (F, 77)
EX-SeV-CW50176 Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (M, 74 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived excitatory neurons from Alzheimer's Disease patient (M, 74)
EX-SeV-CW50177 Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (F, 85 yr donor) - Alzheimer's Disease Alzheimer's Disease Cryopreserved human iPSC-derived excitatory neurons from Alzheimer's Disease patient (F, 85)
EX-SeV-CW60130 Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (F, 6 yr donor) - Epilepsy Epilepsy Cryopreserved human iPSC-derived excitatory neurons from Epilepsy patient (F, 6)
EX-SeV-CW60231 Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (M, 6 yr donor) - Epilepsy Epilepsy Cryopreserved human iPSC-derived excitatory neurons from Epilepsy patient (M, 6)
EX-SeV-CW60236 Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (F, 11 yr donor) - Epilepsy Epilepsy Cryopreserved human iPSC-derived excitatory neurons from Epilepsy patient (F, 11)
EX-SeV-CW20026 Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (M, 10 yr donor) - Autism Spectrum Disorder Autism Spectrum Disorder Cryopreserved human iPSC-derived excitatory neurons from Autism Spectrum Disorder patient (M, 10)
EX-SeV-CW20090 Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (F, 18 yr donor) - Autism Spectrum Disorder Autism Spectrum Disorder Cryopreserved human iPSC-derived excitatory neurons from Autism Spectrum Disorder patient (F, 18)
EX-SeV-CW10130 Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (M, 36 yr donor) - Fatty Liver Disease Fatty Liver Disease Cryopreserved human iPSC-derived excitatory neurons from Fatty Liver Disease patient (M, 36)

Frequently Asked Questions

Do I need a license to use Elixirgen’s iPSC-derived cells and/or differentiation reagent kits?

No. The use of differentiated cells or kits provided by Elixirgen Scientific does not require any additional license from other parties for any type of use, except for use in humans or for therapeutic or diagnostic use. Check out our license statement in Resource section for further detail.

What sizes of Quick-Neuron™ Excitatory - Human iPSC-derived Neurons are available?

Off the shelf, we offer small (>1 million viable cryopreserved cells) and large (5x >1 million viable cryopreserved cells) sizes. If you are looking for a larger quantity, please contact us for pricing information.

Should I use an mRNA- or SeV-based differentiation kit?

Both delivery methods provide robust “footprint-free,” non-integrating expression of the transcription factors, although each kit uses a slightly different workflow. Based on our data, the differentiation efficiency between an mRNA-based and a SeV-based kit is similar. For example, the percentages of TUBB3+ neurons differentiated using Quick-Neuron™ Cholinergic mRNA- and SeV-based kits were 93% and 88%, respectively.

We recommend the mRNA-based neuron differentiation kits for labs with experience culturing iPSCs/ESCs, as the workflow involves slightly more manipulation of fragile differentiating cells than the SeV-based kits. The SeV-based kits are better for labs with little experience culturing iPSCs/ESCs, although they must be used with BSL-2 safety measures and may require prior approval from your Institutional Biosafety Committee (IBC).

What sizes of Quick-Neuron™ differentiation kits are available?

Off the shelf, we offer small and large iPSC neuronal differentiation kits. The small size is best suited for single cell, imaging analyses, and pilot studies while the large size is best for applications such as high throughput screening and RNA sequencing.