Discover the forefront of iPSC neuron research and applications at Elixirgen Scientific. Our cutting-edge iPSC-derived excitatory neurons offer unparalleled insights into neuronal behavior and disease modeling.
Characterization of iPSC-derived excitatory neurons is crucial to ensure their utility in research. Employing excitatory neuron markers, researchers can confirm the identity and purity of these neurons.
Explore our detailed iPSC neuron differentiation protocols, a step-by-step guide designed to simplify and optimize your laboratory procedures using our iPSC-derived cells and differentiation kits. These protocols leverage the latest advancements in iPSC technology to ensure efficient and reproducible results.
Differentiation kit workflow (upper: Large kit, lower: Small kit)
Representative images of Quick-Neuron™ Excitatory - SeV Kit cell cultures on days 1-10 post-differentiation (scale bar = 100 μm).
iPSC-derived cells workflow
Representative images of Quick-Neuron™ Excitatory - Human iPSC-derived Neurons on days 1-7 post-thaw (scale bar = 100 μm).
Understanding the role of excitatory neuron markers is crucial in neuroscience research. Our comprehensive guide delves into the identification and significance of these markers in iPSC-derived neurons, providing essential information for researchers.
iPSC-derived excitatory neurons express neuronal markers and display typical neurite growth. Immunofluorescent staining of Quick-Neuron™ Excitatory - Human iPSC-derived Neurons on Day 7 post-thaw that shows expression of the pan-neuronal marker TUBB3 and the glutamatergic neuron marker vGLUT1 (scale bar = 100 μm).
Electrophysiological properties of iPSC-derived excitatory neurons. Whole-cell patch clamp of Quick-Neuron™ Excitatory - Human iPSC-derived Neurons 5-6 weeks post-thaw. (A) A brightfield image of the neurons measured. (B) Spontaneous action potentials were recorded. (C) Spontaneous excitatory postsynaptic current of neurons was detected by voltage clamp measurement at -70mV, indicating the formation of mature synapses. Data courtesy of E-PHY SCIENCE SAS.
Excitatory Neurons seeded onto MEA plates remain functional after transport. Baseline readings of the same well (A) on day 49 post-thaw before transport and (B) on day 50 after 33 hr transport of an MEA plate seeded with Quick-Neuron™ Excitatory Neurons and primary human astrocytes from Tokyo, Japan to Maryland, USA. (C) Response on day 51 to varying concentrations of 4-AP after transport. Data courtesy of Ricoh.
Gene Expression of Quick-Neuron™ Excitatory Neurons. A heat map of selected gene expression data from RNA-seq performed on undifferentiated iPSCs, Quick-Neuron™ Excitatory Neurons (EX only) cultured for 10 days and 38 days, Quick-Neuron™ Excitatory Neurons cocultured with primary astrocytes (EX+AST) for 18 and 52 days, and primary fetal and adult brain samples is shown. Values represent log10(TPM + 1). Data courtesy of Ricoh.
Quick-Neuron™ Excitatory Neurons display gene expression profiles similar to those of human brain: RNA-seq was performed on undifferentiated iPSCs, Quick-Neuron™Excitatory Neurons (EX only) cultured for 10 days and 38 days, Excitatory neurons cocultured with primary astrocytes (EX+AST) for 18 and 52 days, and primary fetal and adult brain samples. Principal component analysis indicates that Quick-Neuron™ Excitatory Neurons display gene expression similar to that of human brain, particularly when grown with astrocytes. As cells remain in culture over time they more closely resemble adult human brain cells. Data courtesy of Ricoh.
Our Quick-Neuron™ Excitatory - SeV and mRNA Kits (small and large sizes available) allow researchers to quickly, easily, and efficiently differentiate their iPS or ES cell line of choice into excitatory neurons. The kits utilize temperature sensitive Sendai virus and synthetic messenger RNA (syn-mRNA) to deliver our proprietary cocktail of transcription factors that induce differentiation without leaving a genetic footprint.
| Cat No. | Product Name | Disease StatusDescription |
|---|---|---|
| EX-SeV | Quick-Neuron™ Excitatory - SeV Kit | This kit differentiates human pluripotent stem cells into excitatory neurons in 10 days using Sendai virus. |
| EX-MM | Quick-Neuron™ Excitatory - Maintenance Medium | Medium for long-term maintenance of human iPS/ESC-derived Excitatory neurons. |
Quick-Neuron™ Excitatory - mRNA Kits are also available by request. Please inquire via email!
Elixirgen Scientific’s Quick-Neuron™ Excitatory - Human iPSC-derived Neurons are created using human iPS cells made available through a license from The California Institute for Regenerative Medicine (CIRM).
The CIRM iPS cell repository includes over 1,500 iPS cell lines, so please contact us if you would like excitatory neurons derived from a specific donor cell line. Every lot of iPSCs is differentiated into excitatory neurons using our proprietary transcription factor-based technology that is “footprint-free” and delivers functional, high-quality cells.
| Cat No. | Product Name | Disease StatusDescription |
|---|---|---|
| EX-SeV-CW50065 | Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (Healthy Control - standard) | Healthy Control Cryopreserved human iPSC-derived excitatory neurons from Healthy Control patient |
| EX-SeV-HC | Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (Healthy Control) | Healthy Control Cryopreserved human iPSC-derived excitatory neurons from Healthy Control donor |
| EX-SeV-AD | Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (Alzheimer's Disease) | Alzheimer's Disease Cryopreserved human iPSC-derived excitatory neurons from Alzheimer's Disease patient |
| EX-SeV-EP | Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (Epilepsy) | Epilepsy Cryopreserved human iPSC-derived excitatory neurons from Epilepsy patient |
| EX-SeV-AU | Quick-Neuron™ Excitatory - Human iPSC-derived Neurons (Autism Spectrum Disorder) | Autism Spectrum Disorder Cryopreserved human iPSC-derived excitatory neurons from Autism Spectrum Disorder patient |
Have questions about iPSC neuron differentiation or excitatory neuron markers? Our FAQ section offers in-depth answers to common queries, aiding researchers in their experimental designs.
No. The use of differentiated cells or kits provided by Elixirgen Scientific does not require any additional license from other parties for any type of use, except for use in humans or for therapeutic or diagnostic use. Check out our license statement in Resource section for further detail.
Off the shelf, we offer small (>1 million viable cryopreserved cells) and large (5x >1 million viable cryopreserved cells) sizes. If you are looking for a larger quantity, please contact us for pricing information.
Both delivery methods provide robust “footprint-free,” non-integrating expression of the transcription factors, although each kit uses a slightly different workflow. Based on our data, the differentiation efficiency between an mRNA-based and a SeV-based kit is similar. For example, the percentages of TUBB3+ neurons differentiated using Quick-Neuron™ Cholinergic mRNA- and SeV-based kits were 93% and 88%, respectively.
We recommend the mRNA-based neuron differentiation kits for labs with experience culturing iPSCs/ESCs, as the workflow involves slightly more manipulation of fragile differentiating cells than the SeV-based kits. The SeV-based kits are better for labs with little experience culturing iPSCs/ESCs, although they must be used with BSL-2 safety measures and may require prior approval from your Institutional Biosafety Committee (IBC).
Off the shelf, we offer small and large iPSC neuronal differentiation kits. The small size is best suited for single cell, imaging analyses, and pilot studies while the large size is best for applications such as high throughput screening and RNA sequencing.
