Gain a deeper understanding of the complexities of cell signaling and tease apart variations due to age, gender, and donor variability with skeletal muscle derived from human iPS cells.
Differentiation kit workflow
Timeline of skeletal muscle cell differentiation and maturation. Representative images of Quick-Muscle™ Skeletal - mRNA Kit cell cultures on days 1-6 post-differentiation (scale bar = 100 μm).
Quick-Muscle™ Skeletal cells show typical skeletal muscle cell morphology and express MHC. Immunofluorescent staining of Quick-Muscle™ Skeletal - mRNA cell cultures on day 6 post-differentiation show expression of myosin heavy chain (scale bar = 100 μm).
Our Quick-Muscle™ Skeletal - SeV and mRNA Kits allow researchers to quickly, easily, and efficiently differentiate their iPS or ES cell line of choice into skeletal muscle. The kits utilize temperature sensitive Sendai virus and synthetic messenger RNA (syn-mRNA) to deliver our proprietary cocktail of transcription factors that induce differentiation without leaving a genetic footprint.
| Cat No. | Product Name | Disease StatusDescription |
|---|---|---|
| SM-SeV | Quick-Muscle™ Skeletal - SeV Kit | This kit differentiates human pluripotent stem cells into skeletal muscle cells in 6 days using Sendai virus. |
| SM-MM | Quick-Muscle™ Skeletal - Maintenance Medium | Medium for long-term maintenance of human iPS/ESC-derived skeletal muscle. |
Quick-Muscle™ Skeletal - mRNA Kits are also available by request. Please inquire via email!
No. The use of differentiated cells or kits provided by Elixirgen Scientific does not require any additional license from other parties for any type of use, except for use in humans or for therapeutic or diagnostic use. Check out our license statement in Resource section for further detail.
Off the shelf, we offer small (>1 million viable cryopreserved cells) and large (5x >1 million viable cryopreserved cells) sizes. If you are looking for a larger quantity, please contact us for pricing information.
Both delivery methods provide robust “footprint-free,” non-integrating expression of the transcription factors, although each kit uses a slightly different workflow. Based on our data, the differentiation efficiency between an mRNA-based and a SeV-based kit is similar. For example, the percentages of TUBB3+ neurons differentiated using Quick-Neuron™ Cholinergic mRNA- and SeV-based kits were 93% and 88%, respectively.
We recommend the mRNA-based neuron differentiation kits for labs with experience culturing iPSCs/ESCs, as the workflow involves slightly more manipulation of fragile differentiating cells than the SeV-based kits. The SeV-based kits are better for labs with little experience culturing iPSCs/ESCs, although they must be used with BSL-2 safety measures and may require prior approval from your Institutional Biosafety Committee (IBC).
Off the shelf, we offer small and large iPSC neuronal differentiation kits. The small size is best suited for single cell, imaging analyses, and pilot studies while the large size is best for applications such as high throughput screening and RNA sequencing.
