Discovery of induced pluripotent stem cell (iPSC) reprogramming and differentiation offers unprecedented opportunities in disease modeling and drug discovery in stem cell research. Elixirgen Scientific can help accelerate your research using our proprietary technology to provide fast and reliable differentiation of induced pluripotent stem cells (iPSC) for limitless applications. Human iPSCs revolutionize the study of human biology and your lab can access the power with our services. Using our iPSC services enables researchers to harness the benefits of the latest biomedical research tools.
Custom iPSC Differentiation Service
Benefits with Quick-Tissue Technology
At the heart of our services lies the Quick-Tissue technology. This proprietary technique enables efficient and reliable differentiation of iPSCs into various cell types. Our custom iPSC differentiation service employs this technology, allowing researchers to have easy access to high-quality differentiated cells for their experiments by our internal iPSC experts. These differentiated cells can be used in a multitude of applications, including disease modeling, biomarker detection, and drug testing.
Advantages
- Rapid differentiation
- Functionally validated
- Reproducible
- Scaleable
How Our Differentiation Service Works
STEP 1
Project Design Consultation
Whether you’re an experienced iPSC researcher or just getting started, our scientists are here to answer your questions and provide ongoing support. All iPSC services begin with a free consultation to define the scope of the project, including the desired differentiated cell type, the production scale (1-50 million viable cells), and desired characterization assays (ICC, MEA, etc.).
If you don’t currently have an iPSC line, we can also provide assistance in obtaining iPSC lines from iPSC banks or gene-editing service providers. Consultations are free, so request a consultation today to discuss your project requirements. iPSC lines Elixirgen recognize are listed here.
STEP 2
iPSC Expansion or Adaptation (Optional)
Upon receiving your frozen or live iPSC line(s), we expand or adapt the cells in optimal conditions for growth and differentiation. For our transcription factor-based differentiation platform, we use feeder-free, single-cell passaging conditions for iPSC expansion/adaptation for best results.
During iPSC expansion, we can produce working and back-up stocks of the iPSC line along with a certificate of analysis to verify pluripotency, karyotype, and any kind of iPSC assay upon request.
STEP 3
iPSC Differentiation
Our proprietary iPSC differentiation technology produces functional, highly pure populations neurons in as little as 1 week without leaving a genetic footprint.
Our standard differentiated cell types include a variety of neuronal subtypes (excitatory, GABAergic, dopaminergic, cholinergic, or sensory) and astrocytes but we can also use our iPSC expertise to develop a custom differentiation strategy for generating your desired cell or tissue type.
STEP 4
Characterization
Prior to shipment or additionally requested assays, we conduct a variety of quality control checks to ensure that differentiated cells are free from contaminants, express lineage-specific markers, and have optimal post-thaw viability.
We also offer a variety of optional in-house assay services, including microelectrode array (MEA), neurite outgrowth assay, and calcium transient assay, if in depth functional characterization is desired.
Cryopreserved differentiated cells or their derivatives such as RNA and supernatant are shipped depending on preference. Cryopreserved differentiated cells are supplied with media supplements and detailed instructions for optimal cell maintenance.
Questions? Comments? Ask Us
Frequently Asked Questions
How much do the iPSC services cost?
The price for the differentiation of one hPSC (iPS or ES) line varies based on multiple factors and requirements such as iPSC expansion requirement, number of cells required, QC criteria etc. Contact us to share your requirements and start discussion.
I don't have my own cells, where can I order an iPSC line?
If you do not already have an established iPSC line, you can find patient iPSC lines in your interest here. Contact us for you to learn more about sourcing iPSC lines and differentiation.
How do I ship my hPSC line?
Sending biomaterials is always challenging. However, Elixirgen Scientific has a lot of experience in cross border logistics for cells and biological materials. Our knowledge and expertise will navigate you to properly prepare your iPSCs to ship. We use dry ice shipment in the US and liquid nitrogen dry shipper internationally to ship cryopreserved cells. We can also accept live hPSC lines to speed up an entire process and lower total fees.
Can you reprogram my cells into iPSCs?
We do not currently offer services for reprogramming cells into iPSCs. However, we can work with our partners to take care of this reprogramming services from fibroblast or PBMC.
List of diseases and mutations.
Transform how you study disease with Quick-Tissue™ technology
With Quick-Tissue™ technology, you can study disease state from multiple angles in vitro. Thanks to advance in iPSC reprogramming technology, there are thousands of iPSC lines available for your disease study. Look through the iPSC line database below to find out your diseases in interest exist. The database also lists identified mutant genes. Feel free to reach us to how we can help getting differentiated cells derived from iPSC lines in your interest.
Disease Mutant genes (# of iPSC lines) Number of total patient iPSC lines
ABCA1 heterozygous ABC1 (2) 2
Abetalipoproteinemia MTP (2) 2
Acromesomelic dysplasia NPR2 (1) 1
Acute encephalopathy with biphasic seizures and late reduced diffusion (AESD) 1
Adrenoleukodystrophy (ALD) 1
Adult-onset Still’s disease (AOSD) 1
Age-related macular degeneration (AMD) 121
Aicardi syndrome 1
Alexander disease GFAP (3) 6
Allergic granulomatous angiitis 1
Alzheimer's disease (AD) APOE (10), APOE4 (3), APP (4), C9ORF (1), CD33 (2), MAPT (2), PSEN1 (14), PSEN2 (1), TBK1 (1), TREM2 (3) 180 (Available Differentiated cells)
Alzheimer's disease (AD) (Gene-edited) APP (6), PSEN1 (8) 14
Alzheimer's disease (AD) (familial) APP (3), APPV7171 (4), PSEN1 (1), PSEN2 (1) 11
Amyotrophic lateral sclerosis (ALS) ASYMPTOMATIC C9ORF72 CARRIER (1), C9ORF72 (46), FIG4 (1), FUS (3), SETX (1), SETX, SOD1 (1), SOD1 (36), SOD1 > D90A (1), TARDBP (5), VCP (1) 532
Anemia (phenotype) 1
Angelman syndrome UBE3A (2) 10
Aplastic anemia 3
Arrhythmogenic right ventricular cardiomyopathy 2
Arteriolosclerosis 2
Associated pulmonary arterial hypertension 18
Ataxia-telangiectasia 3
Atrial fibrillation 14
Atrial tachycardia 1
Autism spectrum disorder (ASD) 110 (Available Differentiated cells)
Autoimmune hemolytic anemia (AHA) / Idiopathic warm (AHA) 1
Bardet-biedl syndrome 22
Batten disease (cln3) CLN3 (23) 23
Batten disease (cln6) 8
Behçet’s disease 2
Beta thalassemia HBB (2) 2
Bethlem myopathy 2
Bilateral frontoparietal polymicrogyria GPR56 (1) 1
Bipolar disorder 30
Blinding eye disease 18
Borderline NASH (fatty liver disease) 2
Breast cancer BRCA1 (3) 3
Brugada syndrome 6
Buerger’s disease 1
Cardiomyopathy 48
Carpal tunnel syndrome 18
Catecholaminergic polymorphic ventricular tachycardia RYR2 (2) 2
Ccanavan Disease ASPA (1) 1
Cchoroideremia (CHM) CHM (1), NGLY1 (1) 4
Cerebral creatine deficiency syndrome 1 (CCDS1) SLC6A8 (1) 1
Cerebral palsy (CP) 19
Cerebrovascular disease 4
Ceroid lipofuscinosis CHM (1), CLN2 (1) 2
Charcot-Marie-Tooth disease FIG4 (1), MFN2 (10), MPZ (2), PMP22 (7), VCP (1) 22
Chromosome 16p11.2 deletion syndrome 5
Chronic inflammatory demyelinating polyneuropathy (CIDP) 2
Chronic myeloid leukemia 1
Congenital disorder of deglycosylation (CDDG) CFTR (1) 1
Congenital heart block 2
Congenital ichthyosis / Ichthyosis syndrome 1
Congenital insensitivity to pain with anhidrosis (CIPA) 2
Congenital myasthenic syndrome GFPT1 (1) 7
Congenital myopathy MTM11 (1) 1
Control C9ORF72 (5), CCR5 (1), GFAP CORRECTED (2), HBB (1), HD (5), HNF1A (1), MECP2 (2), NGN2 (2), SNCA (1), SOD1 > D90A CORRECTED (1), TAF1 VARIANT CORRECTED (4) 25
Coronary artery disease 43
Corticobasal degeneration (CBD） 1
Crohn's disease 3
Crow‐Fukase syndrome 2
Cystic Fibrosis (CF) DMPK (1) 1
Cystinosis 1
DMD DMD (5) 5
DMRV / GNE myopathy 2
Danon disease 1
Definite NASH (fatty liver disease) 32
Diabetes HNF1A (2) 3
Diabetes mellitus 60
Diabetes mellitus type II 12
Diabetes type I 21
Diabetes type II 95
Diabetes type unknown 4
Diabetic retinopathy (DR) 32
Diamond-Blackfan anemia 1
Dilated cardiomyopathy (DCM) 303
Distal Myopathy 3
Down syndrome 47,XX,+21 (4), 47,XY,+21 (3) 8
Dravet syndrome SCN1A (11) 11
Drug-induced liver injury (DILI) 4
Duchenne Muscular dystrophy (DMD) DMD (1) 2
Dystrophia myotonica 1 (DM1) DMPK (1) 1
Ehlers-Danlos syndrome COL3A1 (1) 2
Eosinophilic granulomatosis with polyangiitis (EGPA) 1
Eosinophilic sinusitis 1
Epidermolysis bullosa 1
Epilepsy ALG13 (1), GABRA1 (1), KCNC1 (1), PCDH19 (2), SCN2A (1) 57 (Available Differentiated cells)
Fabry disease 3
Facioscapulohumeral muscular dystrophy 1 (FSHD1) LRIF1 (1) 6
Familial Mediterranean fever 1
Fatty liver disease - steatosis (not NASH) 2
Focal cortical dysplasia (FCD) 2
Focal segmental glomerulosclerosis 20
Fragile X syndrome FMR1 (4) 4
Friedreich ataxia 1 (FRDA) FXN (2) 2
Frontotemporal degeneration C9ORF72 (4), GRN (4), MAPT (10), PGRN (2), VCP (1) 25
Frontotemporal dementia (FTD) C9ORF72 (6), MAPT (15) 30
Frontotemporal lobar degeneration (FTLD) 2
GM1-gangliosidosis 1
Galactosialidosis 1
Gaucher disease GBA (1) 2
Giant cell arteritis (GCA) 2
Glaucoma 20
Glut1 deficiency syndrome 1 (Glut1DS1) SLC2A1 (1) 1
Glycogen storage disease / GSD type V (muscle glycogen phosphorylase deficiency) 1
Glycosylphosphatidylinositol(GPI) anchor deficiency 3
Granulomatosis with polyangiitis (GPA) 1
Hemiconvulsion-hemiplegia-epilepsy syndrome 2
Hemimegalencephaly 1
Hepatitis C (HCV) 91
Hereditary dystonia 1
Homozygous familial hypercholesterolemia LDLR (6) 6
Hunter syndrome 2
Huntington's disease (HD) HD (14), HTT (36), IT15 (1), SMN1 (1) 58
Hurler syndrome IDUA (1) 1
Hutchinson-gilford progeria syndrome (HGPS) 2
Hyperalphalipoproteinemia SR-BI (2) 2
Hypertrophic cardiomyopathy TNNT2 (1) 83
Idiopathic aplastic anemia 1
Idiopathic pulmonary arterial hypertension 16
Idiopathic pulmonary fibrosis (IPF) 182
Idiopathic thrombocytopenic purpura 1
IgG4-related disease 1
IgG4-related thyroid disease 1
Inappropriate sinus tachycardia 1
Infantile neuroaxonal dystrophy (INAD) PLA2G6 (5) 5
Intellectual disability (ID) 60
Interstitial lung disease 1
Isaacs syndrome 1
Isogenic control 3
Kearns-sayre syndrome (KSS) 1
Keratoconus 2
Krabbe disease GALC (1) 1
Landau-Kleffner syndrome 1
Left ventricular hypertrophy 15
Left ventricular non-compaction cardiomyopathy 10
Lennox-Gastaut syndrome (LGS) 2
Lesch-nyhan syndrome (LNS) HPRT1 (1) 1
Lewy body dementia 8
Lewy body dementia (LBD) 1
Limb-girdle muscular dystrophy DYSF (5) 5
Limb-girdle muscular dystrophy (LGMD2b) DYSF (15) 15
Lissencephaly DCX (1) 1
Long QT syndrome 3
Long QT syndrome (familial) 13
Long QT syndrome 1 (LQT1) 3
Long QT syndrome 2 (LQT2) KCNH2 (1) 1
Long QT syndrome 3 (LQT3) SCN5A (1) 1
MELAS 1
Malignant rheumatoid arthritis (MRA) 1
Mental illness DISC1 EXON 8 WILD-TYPE (2) 2
Mental retardation CHAMP1 (2), SYNGAP1 (1) 3
Mesial temporal lobe epilepsy with hippocampal sclerosis 2
Microscopic polyangiitis (MPA) 1
Migraine disorder MAJOR CHR17 AMPLIFCATION; MINOR CHR7 DELETION (1) 28
Mild left ventricular hypertrophy 1
Miller-dieker lissencephaly syndrome (MDLS) 1
Mitochondrial diseases 1
Mixed Connective-Tissue Disease (MCTD) 1
Monogenic diabetes 13
Mortor dominant 1
Moyamoya disease 3
Mucopolysaccharidosis (MPS) SGSH (1) 3
Multifocal motor neuropathy (MMN) 1
Multiple sclerosis (MS) 4
Multiple system atrophy (MSA) 6
Muro disease (Kii ALS/PDC) 3
Muscular dystrophy DMD (5), LAMA2 (1), POMT2 (1) 9
Myasthenia Gravis (MG) 1
Myocardial infarction 18
Myoclonic epilepsy CHD2 (1) 1
Myotonic dystrophy CNBP (4), DMPK (1) 9
Nescav syndrome KIF1A (5) 6
Neurodegeneration with brain iron accumulation 5 (NBIA5) WDR45 (1) 1
Neurodevelopmental disorder DHPS (1) 1
Neuroferritinopathy 1
Neurofibromatosis type1 (NF1) 1
Neurofibromatosis type2 (NF2) 1
Neuromyelitis Optica 4
Neuromyelitis Optica Spectrum Disorders (NMOSD) 1
Neuronal migration disorder PIK3R2 (1) 3
Neuropathy GARS (2), SCN9A (6) 39
Niemann-pick disease NPC1 (1), SMPD1 (3) 5
Non-als motor neurone disease 1
Ohtahara syndrome STXBP1 (1) 1
Ornithine transcarbamylase deficiency (OTCD) 1
Osteogenesis imperfecta type iv (OI4) COL1A2 (1) 1
PACS1 (Schuurs-Hoeijmakers) syndrome PACS1 (2) 2
Pain agnosia SCN11A (2) 2
Parkinsonism GBA (2), LRRK2 (6), MAPT (1), PARK2 (4), PINK1 (1), SNCA (3) 26
Parkinson’s disease (PD) GBA (18), LRRK2 (8), SNCA (9) 99
Paroxysmal nocturnal hemoglobinuria (PNH) 1
Pemphigoid (including epidermolysis bullosa acquisita) 2
Pemphigus 2
Periodic paralysis 1
Phenylketonuria PAH PAH (1) 2
Pick's disease 1
Pitt-hopkins syndrome (PTHS) TCF4 (1) 1
Polyarteritis nodosa (PAN) 2
Polymicrogyria 1
Pompe’s disease (adult type) 1
Primary antiphospholipid syndrome 2
Primary erythromelalgia SCN9A (2) 4
Primary immunodeficiency syndrome 3
Primary lateral sclerosis (PLS) 7
Primary open angle (POAG) 20
Primary progressive aphasia (PPA) 1
Progressive multifocal leukoencephalopathy (PML) 1
Progressive supranuclea palsy (PSP) 1
Prolonged QT interval 6
Pulmonary arterial hypertension ALK1 (2), BMPR2 (4) 6
Pulmonary atresia 1
Pustular psoriasis 1
Pyogenic sterile arthritis / Pyoderma gangrenosum and acne syndrome 1
Rasmussen encephalitis 2
Relapsing polychondritis (RP) 1
Resolved systolic anterior motion 1
Restrictive cardiomyopathy 1
Retinitis pigmentosa 5
Rett syndrome FOXG1 (5), MECP2 (6), SHANK3 (1) 15
Right ventricular outflow tract premature ventricular contractions 2
Ring chromosome 20 syndrome 2
Sanfilippo syndrome / MPS IIIC (acetyl-CoA:heparan-α-Ｄ-glucosaminide N-acetyltransferase deficiency) 1
Schizophrenia 4
Semantic Dementia 2
Severe combined immunodeficiency ADA (2) 2
Sickle cell anemia HBB (55) 55
Sjögren’s syndrome 1
Skeletal displasia 4
Small atrial septal defect 1
Smith-magenis syndrome (SMS) 1
Spinal muscular atrophy SMN1 (14) 18
Spinal-Bulbar Muscular Atrophy (SBMA) 10
Spinocerebellar Degeneration 14
Spinocerebellar ataxia type 1 2
Spinocerebellar ataxia type 3 ATXN3 (4) 4
Spondylometaphyseal displasia 2
Stevens-Johnson syndrome (SJS) 1
Sturge-Weber syndrome 1
Subacute sclerosing panencephalitis (SSPE) 2
Syringomyelia 1
Systemic amyloidosis 2
TNF receptor-associated periodic syndrome 1
Takayasu arteritis 3
Tangier disease ABC1 (2), ABCA1 (4) 6
Tay-sachs disease (TSD) HEXA (1) 1
Thrombotic thrombocytopenic purpura (TTP) 1
Tricuspid atresia 1
Tuberous sclerosis TSC2 (2) 3
Ventricular tachycardia 5
Vertebrobasilar insufficiency(VBI) 1
Vici syndrome (VICIS) EPG5 (1) 1
Werner syndrome 1
West syndrome 1
Wilson’s disease 4
Wolfram syndrome 1
Wolman disease 1
X-linked creatine transporter deficiency 1
X-linked dystonia Parkinsonism TAF1 VARIANT (34) 34
Xeroderma pigmentosum 2
