Neuron Differentiation Kits & Media

Our Quick-Neuron™ iPSC neuronal differentiation kits allow researchers to quickly, easily, and efficiently differentiate their desired iPS cell line into a variety of neuronal cell types, including excitatory, GABAergic, dopaminergic, and cholinergic neurons.

Quick-Neuron™ Differentiation Kits & Media

Although human iPSCs have many advantages over existing models, a drawback is that differentiation has historically been a very time and labor intensive process. Our Quick-Neuron™ neuron differentiation kits overcome this obstacle by utilizing our proprietary transcription factor-based technology that allows for rapid and reproducible differentiation of stem cells into a variety of neuronal cell types in just 1-2 weeks. Maintenance media for all differentiation kits are also available for long-term maintenance of differentiated neuronal cell cultures.

Advantages

  • Differentiate your desired iPSC line
  • Fast differentiation and maturation
  • Highly pure population
  • No genetic footprint
Cat No. Product Name Disease StatusDescription
EX-SeV Quick-Neuron™ Excitatory - SeV Kit This kit differentiates human pluripotent stem cells into excitatory neurons in 10 days using Sendai virus.
EX-MM Quick-Neuron™ Excitatory - Maintenance Medium Medium for long-term maintenance of human iPS/ESC-derived Excitatory neurons.
GA-mRNA Quick-Neuron™ GABAergic - mRNA Kit This kit differentiates human pluripotent stem cells into GABAergic neurons in 10 days using synthetic mRNA.
GA-MM Quick-Neuron™ GABAergic - Maintenance Medium Medium for long-term maintenance of human iPS/ESC-derived GABAergic neurons.
DA-mRNA Quick-Neuron™ Dopaminergic - mRNA Kit This kit differentiates human pluripotent stem cells into dopaminergic neurons in 10 days using synthetic mRNA.
DA-MM Quick-Neuron™ Dopaminergic - Maintenance Medium Medium for long-term maintenance of human iPS/ESC-derived dopaminergic neurons.
CH-SeV Quick-Neuron™ Cholinergic - SeV Kit This kit differentiates human pluripotent stem cells into cholinergic neurons in 6 days using Sendai virus.
CH-mRNA Quick-Neuron™ Cholinergic - mRNA Kit This kit differentiates human pluripotent stem cells into cholinergic neurons in 7 days using synthetic mRNA.
CH-MM Quick-Neuron™ Cholinergic - Maintenance Medium Medium for long-term maintenance of human iPS/ESC-derived cholinergic neurons.
SS-mRNA Quick-Neuron™ Sensory - mRNA Kit This kit differentiates human pluripotent stem cells into sensory neurons in 10 days using synthetic mRNA.
SS-MM Quick-Neuron™ Sensory - Maintenance Medium Medium for long-term maintenance of human iPS/ESC-derived Sensory neurons.

Characterization

iPSC-derived neurons express neuronal markers and display typical neurite growth. Immunofluorescent staining of cell cultures differentiated using our Quick-Neuron™ differentiation kits on days 7-10 post-differentiation. Neuronal cell cultures show expression of the pan-neuronal marker TUBB3 and subtype specific markers vGLUT1 (glutamatergic), GAD67 (GABAergic), TH (dopaminergic), and ChAT (cholinergic).

Frequently Asked Questions

Do I need a license to commercialize products using Elixirgen’s iPSC neuronal differentiation kits?

No. The use of neuron differentiation kits provided by Elixirgen Scientific does not require any additional license from other parties for any type of use, except for use in humans or for therapeutic or diagnostic use.

Should I use an mRNA- or SeV-based differentiation kit?

Both delivery methods provide robust “footprint-free,” non-integrating expression of the transcription factors, although each kit uses a slightly different workflow. Based on our data, the differentiation efficiency between an mRNA-based and a SeV-based kit is similar. For example, the percentages of TUBB3+ neurons differentiated using Quick-Neuron™ Cholinergic mRNA- and SeV-based kits were 93% and 88%, respectively.

We recommend the mRNA-based neuron differentiation kits for labs with experience culturing iPSCs/ESCs, as the workflow involves slightly more manipulation of fragile differentiating cells than the SeV-based kits. The SeV-based kits are better for labs with little experience culturing iPSCs/ESCs, although they must be used with BSL-2 safety measures and may require prior approval from your Institutional Biosafety Committee (IBC).

What sizes of Quick-Neuron™ differentiation kits are available?

Off the shelf, we offer small and large iPSC neuronal differentiation kits. The small size is best suited for single cell, imaging analyses, and pilot studies while the large size is best for applications such as high throughput screening and RNA sequencing.