iPSC-derived Neural Precursor Cells

Human induced pluripotent stem cells (iPSCs) are a powerful tool for studying neurons in vitro. Human iPS cells overcome many of the limitations of other popular models, such as immortalized cell lines and primary neural precursor cells, which can be plagued by reproducibility issues and lack of biological relevance. Although human iPS cells have many advantages over existing models, one drawback is that differentiation into the desired cell type is a time and labor-intensive process. To address this research bottleneck, Elixirgen Scientific provides a variety of products and services to make research more efficient. Our proprietary technology allows for rapid, reproducible differentiation of human iPSC-derived neural precursor cells without sacrificing purity. 

Advantages

  • Fast differentiation and maturation
  • Highly pure population
  • No genetic footprint

Applications

  • Basic research
  • Drug screening
  • Disease modeling

iPSC-derived Neural Precursor Cells from Healthy and Affected Donors

Elixirgen Scientific’s human iPSC-derived neural precursor cells are created using human iPS cells made available through a license from The California Institute for Regenerative Medicine (CIRM). All of our iPSC-derived excitatory neurons are differentiated using our proprietary technology that utilizes a potent cocktail of key transcription factors to transform iPSCs into high-quality, differentiated excitatory neurons. The CIRM iPS cell repository includes over 1,500 iPS cell lines, so please contact us if you would like neural precursor cells derived from a donor cell line that is not listed in the table below.

Cat no.Donor InformationDisease Status
NP-mRNA-CW50065Human iPSC-derived Neural Precursor Cells (F, 74 yr) Healthy
NP-mRNA-CW10149Human iPSC-derived Neural Precursor Cells (M, 38 yr) Healthy
NP-mRNA-CW20300Human iPSC-derived Neural Precursor Cells (M, 10 yr) Healthy
NP-mRNA-CW50023Human iPSC-derived Neural Precursor Cells (M, 69 yr)Healthy
NP-mRNA-CW70067Human iPSC-derived Neural Precursor Cells (F, 21 yr)Healthy
NP-mRNA-CW50025Human iPSC-derived Neural Precursor Cells (F, 68 yr)Alzheimer’s Disease
NP-mRNA-CW50113Human iPSC-derived Neural Precursor Cells (M, 68 yr)Alzheimer’s Disease
NP-mRNA-CW50114Human iPSC-derived Neural Precursor Cells (F, 72 yr)Alzheimer’s Disease
NP-mRNA-CW50115Human iPSC-derived Neural Precursor Cells (F, 67 yr)Alzheimer’s Disease
NP-mRNA-CW50137Human iPSC-derived Neural Precursor Cells (F, 70 yr)Alzheimer’s Disease
NP-mRNA-CW50147Human iPSC-derived Neural Precursor Cells (M, 71 yr)Alzheimer’s Disease
NP-mRNA-CW60130Human iPSC-derived Neural Precursor Cells (F, 6 yr)Epilepsy
NP-mRNA-CW60231Human iPSC-derived Neural Precursor Cells (M, 6 yr)Epilepsy
NP-mRNA-CW60236Human iPSC-derived Neural Precursor Cells (F, 11 yr) Epilepsy
NP-mRNA-CW20026Human iPSC-derived Neural Precursor Cells (M, 10 yr)Autism Spectrum Disorder
NP-mRNA-CW20090Human iPSC-derived Neural Precursor Cells (F, 18 yr)Autism Spectrum Disorder
NP-mRNA-CW10130Human iPSC-derived Neural Precursor Cells (M, 36 yr)Fatty Liver Disease

Quick-Neuron™ Precursor Differentiation Service

Elixirgen Scientific provides stem cell differentiation services with the world’s fastest turnaround time. Our differentiation method provides homogenous cell cultures without a genetic footprint. Researchers can simply ship live iPSCs to us and receive differentiating or terminally differentiated neural precursor cells in as little as one week. Each project is unique, so please contact us with your specifications to receive more information or to receive a quote.

Characterization

Immunofluorescent staining of Quick-Neuron™ Precursor – Human iPSC-derived NPCs show expression of nestin and vimentin on day 13 post-thaw (scale bar = 100 μm). Staining conditions:  Anti-Nestin Antibody, clone 10C2 (Millipore Sigma, Catalog Number: MAB5326, 1:500 dilution) was used  in combination with a secondary antibody (Invitrogen, Catalog Number: A11032 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 594, 1:500 dilution).  Recombinant Anti-Vimentin antibody [EPR3776] – Cytoskeleton Marker (abcam, Catalog Number: ab92547, 1:500 dilution) was used in combination with a secondary antibody (Invitrogen, Catalog Number: A32731 Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488, 1:500 dilution). Nuclei were counterstained with Hoechst 33342.

Representative phase contrast images of Quick-Neuron™ Precursor – Human iPSC-derived NPCs on days 1-7 post-thaw (scale bar = 100 μm). Cells are ready for passaging at Day 7.